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June 1, 2001AJP Heart and Circulatory Physiology

Gender-specific compensation for the lack of NO in the mediation of flow-induced arteriolar dilation

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Why the study?

Does chronic NO synthesis inhibition with L-NAME alter the mechanisms of flow-induced arteriolar dilation differently in male versus female rats?

Population

Male and female rats (control and chronically treated with N-nitro-L-arginine methyl ester [L-NAME] for 4…

Comparison

Chronic L-NAME treatment for 4 weeks, followed… vs Control rats (no L-NAME treatment)

Design

Preclinical

Follow-up

4 weeks

Key result

Chronic L-NAME treatment preserved flow-induced dilation in both genders, mediated by endothelial prostaglandins in males and a cytochrome P-450 metabolite in females.

Authors

YWYuming WuAHAn HuangDSDong Sun

Discussion

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Overview

Sex-specific endothelial compensation in rats should not alter practice; leaves open human translation of compensatory pathways.

Structured PICO

Does chronic NO synthesis inhibition with L-NAME alter the mechanisms of flow-induced arteriolar dilation differently in male versus female rats?

P
Population
Male and female rats treated with L-NAME for 4 weeks to examine flow-induced dilation of gracilis muscle arterioles.
I
Intervention
Chronic L-NAME treatment for 4 weeks, followed by ex vivo assessment of flow-induced dilation with inhibitors (indomethacin, miconazole, 6-(2-proparglyoxyphenyl)hexanoic acid, charybdotoxin)
C
Comparator
Control rats (no L-NAME treatment)
O
Outcome
Flow-induced dilation of isolated and pressurized gracilis muscle arterioles in response to increases in flow (0-25 microl/min)surrogate

There are significant gender differences in endothelial adaptation to the lack of nitric oxide synthesis, with males relying on prostaglandins and females on cytochrome P-450 metabolites to maintain flow-induced dilation.

Cite This Study

Wu et al. (2001) studied Nitric oxide deficiency. L-NAME vs. Control rats was evaluated on Flow-induced dilation of gracilis muscle arterioles. Chronic L-NAME treatment preserved flow-induced dilation in both genders, mediated by endothelial prostaglandins in males and a cytochrome P-450 metabolite in females.

synapsesocial.com/papers/6a20aee88e09200678d114dahttps://doi.org/10.1152/ajpheart.2001.280.6.h2456
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