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Summary. Glycerolated semen specimens of several donors were examined for DNA content after refrigeration at +6° C for 7 days, and after storage in liquid nitrogen for periods ranging from 2 to 75 weeks. Fresh specimens were also studied. DNA determinations were made in each case by both Feulgen microspectrophotometry (in arbitrary units) and by the Webb-Levy chemical determination. The quantity of DNA per cell in arbitrary units and in mg × 10−9 remained constant after refrigeration, and after freezing-preservation for all time periods studied. Discrepancies between the two kinds of determination, and the concept of embryonic mortality due to aged spermatozoa are discussed.
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ACKERMAN et al. (1968) studied this question.
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