The Organization of the Herpes Simplex Virus Genomes

INTRODUCTION 25 THE HSV DNA . . . . . . ....... . . .... . . .. ... .... ... . . .. . . . . . . . . . . . . . . . . .. . ..... . ... . .. . ... . .. . . .. 26 Conformation, Size, and Base Composition 26 Interruptions in HSV DNA 26 Sequence Arrangement in HSV DNA 27 Isomeric Aangements of HSV DNA 29 Intertypic and Intratypic Variability in HSV DNAs 30 Comparison of HSV DNA with the DNAs of Other Herpesviruses 32 THE FUNCTIONAL ORGANIZATION OF THE HSV GENOME .. . . . . . . . . . . . . . . . . . . . . 35 The Expression of Viral Gene Products in Productively Infected Cel/s 35 ts Mutants of HSV-I and HSV-2 37 Physical Mapping of Viral Genes 39 THE ISOMERIZATION OF HSV DNA ..... ... . . . . . . ... ... .. . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . ....... 42 Functional Equivalence of the Four Isomers of HSV-I DNA 42 The Functional and Structural Equivalence of the Reiterated Sequences in the Land S Components 44 The Origins of Viral DNA Synthesis 47 The Mode of Replication of HSV DNA 48 The Model of Replication of HSV DNA 50