A saturated fat-rich diet enhanced the selective uptake of LDL cholesteryl esters in the arterial wall of mice, a process that correlated with increased plasma cholesterol and arterial lipoprotein lipase levels.
Does a saturated fat-rich diet increase selective uptake of LDL cholesteryl esters in the arterial wall of mice?
A saturated fat-rich diet enhances arterial LDL cholesteryl ester delivery via selective uptake, a process potentially modulated by lipoprotein lipase levels and atherogenesis.
Plasma LDL levels and atherosclerosis both increase on a saturated fat-rich (SAT) diet. LDL cholesterol delivery to tissue may occur via uptake of the LDL particles or via selective uptake (SU), wherein cholesteryl ester (CE) enters cells without concomitant whole-particle uptake. It is not known how dietary fats might directly affect arterial LDL-CE uptake and whether SU is involved. Thus, mice that are relatively atherosclerosis resistant (C57BL/6) or susceptible to atherosclerosis (apoE) were fed a chow or SAT diet and injected with double radiolabeled or fluorescent-labeled human LDL to independently trace LDL-CE core and whole-particle uptake, respectively. Our results show that a SAT diet increased contributions of SU to total arterial LDL-CE delivery in C57BL/6 and apoE mice. The SAT diet increased plasma fatty acid and cholesterol levels; cholesterol, but not fatty acid, levels correlated with SU, as did the degree of atherosclerosis. Increased SU did not correlate with arterial scavenger receptor class B type I levels but paralleled increased lipoprotein lipase (LPL) levels and LPL distribution in the arterial wall. These studies suggest that arterial LDL-CE delivery via SU can be an important mechanism in vivo and that dietary influences on arterial LPL levels and atherogenesis modulate arterial LDL-CE delivery, cholesterol deposition, and SU.
Seo et al. (2005) studied Atherosclerosis. Saturated fat-rich diet vs. Chow diet was evaluated on Contribution of selective uptake to total arterial LDL-CE delivery. A saturated fat-rich diet enhanced the selective uptake of LDL cholesteryl esters in the arterial wall of mice, a process that correlated with increased plasma cholesterol and arterial lipoprotein lipase levels.