Cultures were grown under a constant stream of gas of known composition to eliminate accumulation of any volatiles produced by the fungus. CO 2 -free air completely inhibits colony initiation. Air containing 1% CO 2 allows colony initiation to the same extent as occurs in plugged, nonaerated controls, while "normal air" (ca. 0.03% CO 2 ) causes initiation to be delayed to a variable extent.
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Boasson et al. (1981) studied this question.
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