Mung Bean Nuclease I

Mung bean nuclease I is highly preferential for single stranded (denatured) DNA; the numerical value of this preference depends on the type of DNA and the reaction conditions; at 3'7" in the absence of Mgz+ it is 30,000-fold for T4 DNA, 65-fold for crab d(A-T),, and less than Z-fold for biosynthetic d(A-T),.No detectable hydrolysis of poly dG.poly dC occurs