Myeloid-specific knockout of SHP2 regulates PI3K/PLCγ signaling pathway to protect against early myocardial infarction injury

Figures

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  Figure 1. SHP2MAC-KO improves the cardiac function in mice with myocardial infarction. (A) Ultrasound imaging of mice in sham: SHP2WT group, sham: SHP2MAC-KO group, Model: SHP2WT group and Model: SHP2MAC-KO mice; (B) Statistics of EF, FS, LVAW; d, LVAW; s, LVID; d and LVID; s. P<0.01, ns P>0.05.

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  Figure 2. SHP2MAC-KO reduces myocardial infarct size in mice. (A) TTC results; (B) percentage of infarct size in each group; (C) Plot of Masson Staining result; (D) Myocardial infarction area statistics. **P<0.01, ns P>0.05.

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  Figure 3. SHP2MAC-KO reduces the number of apoptosis and inflammatory cell infiltration in mouse myocardial infarction. (A) Plots of TUNEL staining results and statistics of the number of apoptotic cells; (B) immunohistochemical staining results and relative fluorescence intensity data of TNFα. **P<0.01, ns P>0.05.

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  Figure 4. SHP2MAC-KO can affect the expression of PI3K/PLCγ signaling pathway, the expression of inflammatory factors and proteins related to phagocytic activity. (A) Protein band diagram of p-SHP2, p-PI3K, p-MerTK, p-PLCγ2, p-MyD88, p-NF-κB, TNFα, IFNγ, IL-1β; (B) Relative protein expression of p-SHP2, p-PI3K, p-MerTK, p-PLCγ2, p-MyD88, p-NF-κB, TNFα, IFNγ, IL-1β. **P<0.01.

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  Figure 5. SHP2MAC-KO can mediate the PI3K/PLCγ signaling pathway to influence the expression of inflammatory factors and proteins related to phagocytic activity. (A) Protein band diagram of p-PI3K, p-MerTK, p-PLC Γ2, p-MyD88, p-NF-κb, TNF α, IFN γ, il-1β; (B) Relative protein expression amounts of p-PI3K, p-MerTK, p-PLCγ2, p-MyD88, p-NF-κb, TNF α, IL-1β. **P<0.01, ns P>0.05.

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  Figure 6. Phagocytosis of macrophages detected by phagocytosis assay with fluorescent microspheres. (A) Results of Phagocytosis Assay with Fluorescent Microspheres; (B) Statistics of Phagocytosis Assay with Fluorescent Microspheres. ** P<0.01, ns P>0.05.

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  Figure 7. ELISA detection of the content of inflammatory factors in macrophages in each group. (A) Statistics of the TNF α; (B) Statistics of IL-1 β **P<0.01, ns P>0.05.

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  Figure 8. Myeloid-specific knockout SHP2 mediates the PI3K/PLCγ signaling pathway, affects the expression of inflammatory factors and the level of phagocytic activity, thereby promoting the phagocytic activity of macrophages and reducing the content of inflammatory factors, thereby protecting early myocardial infarction injury.