Modified method improves sensitivity of acid phosphatase measurement, emphasizing standardized enzyme activity assessment.
We have modified a previously described method for determining acid phosphatase, with thymolphthalein monophosphate as substrate, to increase its sensitivity. We assessed effects of serum on variables influencing acid phosphatase activity as measured by this method. The method is shown to be not completely specific for prostatic acid phosphatase. The importance of standardizing methodology in measurement of enzyme activities is demonstrated.
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Ewen et al. (1976) studied this question.
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