Key result
ADP markedly inhibited the actin-activated MgATPase activity and in vitro motility of mouse myosin V, with an ADP dissociation rate of ~11.5 s(-1) indicating it is close to the rate-limiting step.
Population
Recombinant mouse myosin V fragments expressed in Sf9 cells and intact myosin V purified from mouse brain
Design
Preclinical
Authors
Loading...
Preclinical mouse myosin V kinetics warrant caution before clinical translation; extends in vitro understanding of ADP-limited processivity.
The high affinity for actin and slow rate of ADP release allow the myosin V head to remain attached to actin for a large fraction of its ATPase cycle, enabling actin filament movement by only a few molecules.
Wang et al. (2000) studied this question. ADP and Ionic Strength was evaluated on Kinetic and motile properties (MgATPase activity, in vitro motility, ADP dissociation rate). ADP markedly inhibited the actin-activated MgATPase activity and in vitro motility of mouse myosin V, with an ADP dissociation rate of ~11.5 s(-1) indicating it is close to the rate-limiting step.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: