When skinned fibers from frog semitendinous muscle were mildly treated with trypsin, passive tension generation was remarkably reduced, and the hydrolysis of a-connectin to ,8-connectin simultaneously occurred. On immunoelectron microscopy, splitting of the connectin filaments at the A-I junction area in a sarcomere was seen. These observations strongly suggest that connectin filaments are responsible for passive tension generation as a parallel elastic component in a sarcomere.
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Yoshioka et al. (1986) studied this question.