Demonstrates a rapid method for cell-cycle analysis in mammalian cells, highlighting its potential for real-time experiments.
Mithramycin added to mammalian cells fixed in aqueous ethanol is bound to DNA and fluoresces in direct proportion to the cellular DNA content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides a rapid method for cell-cycle analysis and for the first time permits continuous monitoring of cell-cycle kinetics during ongoing experiments.
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Crissman et al. (1974) studied this question.
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