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June 21, 1974Science447 citations

Cell-Cycle Analysis in 20 Minutes

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HCHarry A. CrissmanRTRobert A. Tobey

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Abstract

Mithramycin added to mammalian cells fixed in aqueous ethanol is bound to DNA and fluoresces in direct proportion to the cellular DNA content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides a rapid method for cell-cycle analysis and for the first time permits continuous monitoring of cell-cycle kinetics during ongoing experiments.

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Cite This Study

Crissman et al. (1974) studied this question.

synapsesocial.com/papers/6a20f15be72f0fa382ecbf67https://doi.org/10.1126/science.184.4143.1297
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