Dietary α-linolenic acid delayed arterial thrombus formation in mice compared with a low-ALA diet (P<0.005) and inhibited tissue factor expression and platelet activation.
Does dietary α-linolenic acid reduce arterial thrombus formation and platelet activation in preclinical models?
Dietary α-linolenic acid demonstrates direct antithrombotic effects by impairing arterial thrombus formation, tissue factor expression, and platelet activation in preclinical models.
p-value: p=<0.005
OBJECTIVE: Plant-derived α-linolenic acid (ALA) may constitute an attractive cardioprotective alternative to fish-derived n-3 fatty acids. However, the effect of dietary ALA on arterial thrombus formation remains unknown. METHODS AND RESULTS: Male C57Bl/6 mice were fed a high-ALA or low-ALA diet for 2 weeks. Arterial thrombus formation was delayed in mice fed a high-ALA diet compared with those on a low-ALA diet (n=7; P<0.005). Dietary ALA impaired platelet aggregation to collagen and thrombin (n=5; P<0.005) and decreased p38 mitogen-activated protein kinase activation in platelets. Dietary ALA impaired arterial tissue factor (TF) expression, TF activity, and nuclear factor-κB activity (n=7; P<0.05); plasma clotting times and plasma thrombin generation did not differ (n=5; P=not significant). In cultured human vascular smooth muscle and endothelial cells, ALA inhibited TF expression and activity (n=4; P<0.01). Inhibition of TF expression occurred at the transcriptional level via the mitogen-activated protein kinase p38 in smooth muscle cells and p38, extracellular signal-regulated kinases 1 and 2, and c-Jun N-terminal kinases 1 and 2 in endothelial cells. CONCLUSIONS: ALA impairs arterial thrombus formation, TF expression, and platelet activation and thereby represents an attractive nutritional intervention with direct dual antithrombotic effects.
Holy et al. (Sat,) conducted a other in Arterial thrombus formation. Dietary α-Linolenic Acid (high-ALA diet) vs. Low-ALA diet was evaluated on Arterial thrombus formation (p=<0.005). Dietary α-linolenic acid delayed arterial thrombus formation in mice compared with a low-ALA diet (P<0.005) and inhibited tissue factor expression and platelet activation.