June 23, 2006Circulation Research
Paclitaxel Enhances Thrombin-Induced Endothelial Tissue Factor Expression via c-Jun Terminal NH ₂ Kinase Activation
Key result
Paclitaxel at 10(-5) mol/L elicited a 2.1-fold increase in thrombin-induced tissue factor protein expression and a 1.6-fold increase in surface activity in human endothelial cells.
Why the study?
Does paclitaxel enhance thrombin-induced tissue factor expression in human endothelial cells?
Population
Human endothelial cells
Comparison
Paclitaxel pretreatment followed by thrombin vs Thrombin alone
Design
Preclinical
Authors
Discussion
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Member takes
Implication
May promote paclitaxel-eluting stent thrombosis risk; hypothesis-generating and requires clinical validation.
Structured PICO
Does paclitaxel enhance thrombin-induced tissue factor expression in human endothelial cells?
P
Population
In vitro study evaluating the effect of paclitaxel on tissue factor expression in human endothelial cells.
I
Intervention
Paclitaxel (and docetaxel) pretreatment followed by thrombin
C
Comparator
Thrombin alone
O
Outcome
Tissue factor (TF) protein expression and surface activitysurrogate
Main Result
Effect estimate: 2.1-fold increase
Paclitaxel enhances endothelial tissue factor expression via JNK activation, providing a potential molecular mechanism for stent thrombosis after paclitaxel-eluting stent deployment.
Cite This Study
Stähli et al. (2006) studied this question. Paclitaxel vs. Thrombin alone was evaluated on Tissue factor (TF) protein expression (2.1-fold increase). Paclitaxel at 10(-5) mol/L elicited a 2.1-fold increase in thrombin-induced tissue factor protein expression and a 1.6-fold increase in surface activity in human endothelial cells.