A permanent hybridoma that secretes monoclonal antibody to tubulin has been developed. Monoclonal antibody belongs to the IgG1 subclass and its specificity was determined by enzymoimmunoassay and by indirect immunofluorescence. A cytoplasmic microtubule complex was detected by this monoclonal antibody as demonstrated in three different cell types.
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Viklický et al. (1982) studied this question.
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