Effect of Sphingomyelinase-Treated LDLs on HUVECs

Figures

• 

  Figure 1. The effect of modified LDLs on cell viability in HUVECs. Cell viability was evaluated in HUVEC cells incubated for 24 h in the absence or presence of control LDLs (C-LDLs), oxidized LDLs (ox-LDLs) and LDLs treated with 50 mU/mL or 100 mU/mL SMase (SMase-LDLs). Results are presented as mean ± SD of 3 determinations carried out in triplicate. (* p < 0.05 vs. cells incubated with C-LDL).

• 

  Figure 2. The effect of modified LDLs on Caspase-3 in HUVECs. (A) Densitometric analysis of Caspase-3 in HUVEC cells incubated for 24 h in the absence or in the presence of control LDLs (C-LDLs), oxidized LDL (ox-LDLs) and LDLs treated with 100 mU/mL SMase (SMase-LDLs). Data are normalized on β actin. Results are presented as mean ± SD of 3 determinations carried out in triplicate. (* p < 0.05 vs. cells incubated with C-LDL), (B) representative western blot images of caspase-3.

• 

  Figure 3. The effect of modified LDLs on intracellular ROS production in HUVECs. Intracellular ROS production in HUVECs incubated for 24 h in the absence or in the presence of control LDLs (C-LDLs), oxidized LDLs (ox-LDLs) and LDLs treated with 100 mU/mL SMase (SMase-LDLs). Results are presented as mean ± SD of 3 determinations carried out in triplicate. (* p < 0.05 vs. cells incubated with C-LDL).

• 

  Figure 4. The effect of modified LDLs on levels of PON2 and SOD2 in HUVECs. (A) Densitometric analysis and representative western blot images of PON2; (B) relative mRNA levels of SOD2 in HUVEC cells incubated for 24 h in the absence or in the presence of control LDLs (C-LDLs), oxidized LDLs (ox-LDLs) and LDLs treated with 100 mU/mL SMase (SMase-LDLs). Results are presented as mean ± SD of 3 determinations carried out in triplicate. Densitometric data are normalized on Vinculin. (* p < 0.05, ** p < 0.001, **** p < 0.0001 vs. cells incubated with C-LDL).

• 

  Figure 5. Effect of modified LDLs on levels of NF-κB -in HUVECs. (A) Densitometric analysis of NF-κB in HUVEC cells incubated for 24 h in the absence or in the presence of cells incubated with control LDLs (C-LDLs), oxidized LDLs (ox-LDLs) or LDLs treated with 100 mU/mL SMase (SMase-LDLs). Densitometric data are normalized on Vinculin. Results are presented as mean ± SD of 3 determinations carried out in triplicate. (* p < 0.05, **** p < 0.0001 vs. C-LDL). (B) Representative western blot images.

• 

  Figure 6. The effect of modified LDLs on levels of IL-6 and IL-8 in HUVECs. (A) Relative mRNA levels of IL-8, (B) relative mRNA levels of IL-6, and (C) protein levels of IL-6, in HUVECs incubated for 24 h in the absence or in the presence of control LDLs (C-LDLs), oxidized LDLs (ox-LDLs) and LDLs treated with 100 mU/mL SMase (SMase-LDLs). Densitometric data are normalized on Vinculin. Results are presented as mean ± SD of 3 determinations carried out in triplicate. (* p < 0.05, ** p < 0.001, vs. C-LDL).