Probiotics derived from an identical host′s gastrointestinal (GI) system confer superior colonization and provide greater benefits than other commercial sources. This study reports on the probiotic potentials of lactic acid bacteria (LAB) identified from the gut content of Ompok pabda through morphological, physiological, and molecular sequencing for their usefulness in aquaculture. Molecular sequencing confirmed two LAB strains affiliated with the genus Weissella; namely Weissella confusa OPL2 and Weissella cibaria OPL3. Each strain was evaluated for its capability to withstand conditions relevant to feed application and gastrointestinal survival, including fluctuations in temperature, NaCl concentration, pH, and gut bile. Both strains consistently tolerated acidic pH (pH 2–5), bile concentration up to 7.5%, elevated temperature (4°C–45°C), and salt concentration (3%–6%). In addition, the strains demonstrated strong cell‐surface properties, including higher hydrophobicity with time (68.77 ± 0.17 % to 69.36 ± 0.41 % in xylene, 65.62 ± 0.48 % to 67.73 ± 0.36 % in toluene), pronounced autoaggregation capacity (64.15 ± 0.35 % and 64.12 ± 0.53 % ), notable coaggregation rates of 52.79 ± 2.15 % and 53.05 ± 0.35 % with Bacillus aerius , and 50.11 ± 0.16 % and 49.51 ± 0.81 % with Staphylococcus epidermidis , for W. cibaria OPL3 and W. confusa OPL2, respectively. Furthermore, the strains showed marked in vitro antagonism against Aeromonas veronii OPP8 and Plesiomonas shigelloides OPP9. Safety assessment further supported their suitability as probiotics, as they were found nonhemolytic in nature, as well as susceptible to several commonly used antibiotics. Therefore, the findings indicate W. cibaria OPL3 and W. confusa OPL2 as a safe and promising probiotic candidate from O. pabda with clear probiotic potentials in the aquaculture industry.
Rifa et al. (Thu,) studied this question.