Barcoding for Isobaric Tagging of Carboxylic Acids and Amines Metabolites

Key Points

• This research aims to expand the detection range of metabolites through innovative isobaric tagging techniques.
• Developed a barcoding approach for simultaneous labeling of carboxylic acids and amines.
• Utilized advanced analytical techniques to assess metabolomic profiles.
• Conducted comparative analyses against conventional labeling methods.
• Increased the range of detectable metabolites by 40% compared to traditional methods.
• Demonstrated improved quantification accuracy with a p-value of <0.05.
• Achieved successful tagging of both primary amines and carboxylic acids in single injections.