Randomized trial investigates exosomal microRNA changes to understand cardiac recovery in Ebstein's anomaly, suggesting novel biomarkers.
Key Points
This study aims to uncover the molecular mechanisms of cardiac repair after cone reconstruction in Ebstein’s anomaly patients through exosomal microRNA profiling.
Collected plasma samples from 10 EA patients before and after cone reconstruction and from 10 healthy controls.
Isolated plasma exosomes using size-exclusion chromatography and sequenced exosomal miRNAs.
Conducted differential expression, functional enrichment, time-series clustering, and correlation analyses with clinical parameters.
Identified distinct exosomal miRNA signatures in EA patients preoperatively, linked to cardiac development and apoptosis.
MiRNAs related to inflammation and myocardial stress were upregulated on postoperative day 1.
By day 7, molecular pathways indicated structural remodeling and functional recovery, with specific miRNAs correlating with cardiac function metrics.