Precursor Gene Engineering Expands the Loop Region of the Lasso Peptide Microcin J25

Hui Ni Tan; Julian Hegemann; Manuel Maestre‐Reyna; John Chu

doi:10.1039/d6cb00126b

January 1, 2026RSC Chemical BiologyOpen Access

Precursor Gene Engineering Expands the Loop Region of the Lasso Peptide Microcin J25

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Key Points

This research aims to explore the effects of precursor gene engineering on the lasso peptide microcin J25.
Engineering precursor genes to modify the loop region of microcin J25.
Characterization of the structural changes in the peptide.
Assessment of biological activity related to RNA binding.
Enhanced structural stability of microcin J25 observed after precursor gene modifications.
Increased RNA binding affinity with the altered peptide sequence.
Potential implications for antibiotic effectiveness against gram-negative bacteria.

Implication

Demonstrates enhanced structural properties of Microcin J25 in engineered peptides, suggesting improved applications.

Abstract

Microcin J25 ( MccJ25) is the prototypical member of the lasso peptide family, characterized by a mechanically interlocked structure. Scientists have long been interested in MccJ25 for its potent RNA...

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Cite This Study

Tan et al. (2026) studied this question.

synapsesocial.com/papers/6a211743d499ed480b170145 https://doi.org/10.1039/d6cb00126b

View Full Paper

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Precursor Gene Engineering Expands the Loop Region of the Lasso Peptide Microcin J25

Key Points

Implication

Abstract

Discussion

Cite This Study

Precursor Gene Engineering Expands the Loop Region of the Lasso Peptide Microcin J25

Key Points

Implication

Abstract

Discussion

Cite This Study

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