Cold and Rewarming Alter Polyunsaturated Fatty Acid Profiles in Silver Pomfret via Regulation of Elongation of Very Long-Chain Fatty Acids (Elovl)

responded strongly to temperature change, showing marked upregulation during cooling and a recovery-related shift during rewarming. Molecular docking suggested its preference for C18-C20 acyl-CoA substrates. Lipidomics revealed that cold exposure followed by rewarming increased PUFA levels by >8% in fish muscle, especially EPA and DHA. Collectively, these findings suggest that cold-rewarming induces beneficial PUFA remodeling associated with Elovl regulation and may provide a practical strategy for improving the nutritional quality of aquaculture fish.