Redox Status-Selective Imaging of Iron in Vegetative and Pathogenic Fungal Cells Using Fluorescent Dyes Synthesized Via Simple Chemical Reactions

Iron plays a prominent role in various biological processes and is an essential element in almost all organisms, including plant-pathogenic fungi. As a transition element, iron occurs in two redox states, Fe 2+ and Fe 3+ , the transition between which generates distinct reactive oxygen species (ROS) such as H 2 O 2 , OH − anions, and toxic OH· radicals. Thus, the redox status of Fe determines ROS formation in pathogen attack and plant defense and governs the outcome of pathogenic interactions. Therefore, spatially resolved visualization of Fe 2+ and Fe 3+ are essential to understand microbial pathogenesis. Here, we report a simple method for synthesis of the redox-state-selective dyes pyrene-tetramethyl piperidinyl oxyl (p-TEMPO) and 4-(4-methylpiperazine-1)-7-nitrobenz-2-oxa-1,3-diazole (MPNBD) for fluorescence microscopy-based imaging of Fe 2+ and Fe 3+ ions. Using these dyes, the occurrence and spatial distribution of Fe 2+ and Fe 3+ ions in vegetative and pathogenic hyphae of the hemibiotrophic maize anthracnose fungus Colletotrichum graminicola are shown. [Formula: see text]