Serum Lipid and Protein Fractions

Ninety-six patients were observed at intervals for as long as three and one-half years in some cases. In most instances multiple determinations were made during this time using the electrophoretic pattern of the proteins before and after cold ether extraction (McFarlane method), to measure lipid transport. Hyperlipemia usually produced an increased in beta globulin. A varying decrease in albumin accompanied any increase in globulin. Phosphate and barbiturate buffers were used in this study. In setting up a control group, it was noted that the sera from professional blood donors contained relatively less albumin and more globulin than the sera of the nondonor group of hospital personnel.