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β-1,4-Mannanases are of common occurrence in fungi. They are induced enzymes, the yields of enzyme being 10- to 100-fold greater when the organism is grown on mannan. All of the β-mannanases studied are endopolysaccharases acting on long chains in a random manner and unable to act on mannotriose or mannobiose. Acting on substituted mannans, they yield products containing the branch. Most of the microbial mannanase preparations contain glycosidases capable of removing galactose or glucose units from the mannan chain. The few which lack glycosidases are useful in the production of oligosaccharide products.
Reese et al. (Thu,) studied this question.