In order to characterize hybrid bio/abio technology utilizing carbon nanotubes (CNTs), in situ, real-time, yet noninvasive methods of accurate and reliable imaging are needed for observing CNTs’ interactions with biological materials, i.e., DNA, in biologically relevant aqueous environments. Optical visualization and characterization of individual CNTs in aqueous solutions were explored in this study using 1-pyrenebutanoic acid, succinimidyl ester (PSE) and a conventional fluorescence microscope. The results demonstrate the potential of fluorescence microscopy based on PSE-based staining methodology monitoring with nanometer resolution of individual CNTs and their manipulation with biological materials in bio/abio hybrid systems.
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Kim et al. (2006) studied this question.
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