DNA prepared from adenoviruses by treatment with pronase and sodium dodecyl sulfate (SDS) and extraction with phenol consists of linear, non-permuted, duplex molecules having inverted terminal repetitions (van der Eb and van Kesteren 1966; Green et al. 1967; Doerfler and Kleinschmidt 1970; Laver et al. 1971; Younghusband and Bellett 1971, 1972; Garon et al. 1972; Wolfson and Dressler 1972). The 5′ ends possibly have short, noncomplementary single-stranded regions (J. Sussenbach, pers. comm.). When replicating DNA is extracted from adenovirus-infected cells by a similar method, it consists of linear, genome-length molecules with extensive single-stranded regions; no circles or concatemers with continuous strands can be detected (Bellett and Younghusband 1972; Sussenbach et al. 1972; van der Eb 1973). Four main classes of replicating molecules are found: forked molecules with one arm single stranded; similar molecules with one arm partly single stranded; unbranched molecules partly single stranded and partly duplex; and unbranched, mainly...
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Robinson et al. (1974) studied this question.