Purification of a human prostate specific antigen

No AccessJournal of Urology1 Feb 2002Purification of a human prostate specific antigen M.C. Wang, L.A. Valenzuela, G.P. Murphy, and T.M. Chu M.C. WangM.C. Wang More articles by this author , L.A. ValenzuelaL.A. Valenzuela More articles by this author , G.P. MurphyG.P. Murphy More articles by this author , and T.M. ChuT.M. Chu More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(02)80311-1AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Rabbit antiserum raised against the crude extract of normal human prostatic tissue contained antibodies to a prostatic tissue-specific antigen as shown by immunoprecipitation techniques. Using this antiserum a prostate antigen was detected in normal, benign hypertrophic and malignant prostatic tissues, but not in other human tissues. The prostate antigen was purified to homogeneity from prostatic tissues and showed a single protein band on analytical polyacrylamide gel electrophoresis and isoelectric focusing This report thus presents the first demonstration of the purification of a prostate-specific antigen that does not represent prostatic acid phosphatase. References 1. : Carcinoma of the prostate in elderly men: incidence, growth characteristics and clinical significance. J. Urol.1969; 101: 602. Link, Google Scholar 2. : An “acid” phosphatase occurring in the serum of patients with metastasizing carcinoma of the prostate gland. J. Clin. Invest.1938; 17: 473. Crossref, Medline, Google Scholar 3. : Studies on the antigenic properties of prostate tissue I. J. Urol.1960; 84: 134. Link, Google Scholar 4. : Tissue-and species-specific antigens of normal human prostatic tissue. J Immunol.1970; 104: 1329. Google Scholar 5. : Immunologic studies of normal, benign and malgnant human prostatic tissue. Cancer1972; 29: 1570. Google Scholar 6. : Investigation of specific antigens in prostate cancer. Cancer Chemother. Rep.1975; 59: 105. Google Scholar 7. (Abstract) : Tissue specific and tumor specific antigens in human prostate. Fed. Proc.1977; 36: 1254. Google Scholar 8. : Isolation of Wilm's tumor antigens by chelation. Clin. Chim. Acta1975; 61: 411. Google Scholar 9. : Enzyme markers in human prostatic carcinoma. Cancer Treat. Rep.1977; 61: 193. Google Scholar 10. : Immunochemical detection of serum prostatic acid phosphatase. Methodology and clinical evaluation. Invest. Urol.1978; 15: 319. Google Scholar 11. : A procedure to increase the sensitivity of staining by Coomassie brilliant blue R250-perchloric acid solution. Anal. Biochem.1976; 75: 634. Google Scholar 12. : The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem.1969; 244: 4406. Google Scholar 13. : General remarks of principles, equipment, reagents and procedures. In: A Manual of quantitative Immunoelectrophoresis. Methods and Applications. Edited by . Oslo: Universitetsforlaget1973: 15. Google Scholar 14. : Protein measurement with the Folin phenol reagent. J. Biol. Chem.1951; 193: 265. Crossref, Medline, Google Scholar 15. : An improved acid phosphatase procedure. Clin. Chim. Acta1966; 13: 264. Google Scholar 16. (Abstract) : Purification and characterization of a human prostate tissue-specific antigen. Fed. Proc.1979; 38: 1069. Google Scholar © 2002 by American Urological Association, IncFiguresReferencesRelatedDetails Volume 167Issue 2 Part 2February 2002Page: 960-964 Advertisement Copyright & Permissions© 2002 by American Urological Association, IncMetrics Author Information M.C. Wang More articles by this author L.A. Valenzuela More articles by this author G.P. Murphy More articles by this author T.M. Chu More articles by this author Expand All Advertisement PDF downloadLoading ...