The Primary Structure of the NH2-Terminal Region of Histone T

Histone T is a new histone found in several tissues of the rainbow trout, Salmo gairdnerii (Wigle, D. T., and Dixon, G. H. (1971) J. Biol. Chem. 246, 5636–5644). It has been prepared from trout testis chromatin by extraction with 5% trichloroacetic acid and purified by chromatography on carboxymethylcellulose using a lithium chloride gradient. The sequence of amino acids in the NH2-terminal region has been determined using an automatic protein Sequencer. In its primary structure, histone T differs from any of the other histones yet sequenced, and is thus not a degradation product derived from them.