Gymnopus aquosus (Bull.) Antonin & Noordel and Inocybe subporospora Kuyper were recorded for the first time in Türkiye, among the fungal specimens collected during field studies in Kütahya. The specimens were identified based on morphological, microscopic features and analysis of ITS gene sequences. The habitats and characteristic features of the species are briefly explained and relevant photographs are presented. GenBank accession numbers were assigned to the samples in this study and phylogenetic trees were constructed to compare them with similar taxa. Gymnopus aquosus is mainly characterized by and distinguishes it from related species; have an almost to the center translucently striate, pileus center pale yellow to ochre, pallescent to almost white towards to margin and a distinctly bulbous base with pinkish-chromic rhizomorphs. Inocybe subporospora; have spores with an indistinct germ-pore, red-brown stipe and dark grayish brown pileus with rimulose margin is a very characteristic species that can be distinguished from its close species with its features.
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