Systematic engineering of synthetic serine cycles in Pseudomonas putida uncovers emergent topologies for methanol assimilation

To advance sustainable bioproduction, we engineered Pseudomonas putida for methanol assimilation via modular synthetic serine cycle variants. By combining native and synthetic functions, we enabled methanol-dependent serine biosynthesis and revealed new metabolic topologies, offering a blueprint for C1 assimilation in non-model, industrially relevant bacteria.