Key result
Guan-Xin-Shu-Tong capsule treatment in rats with diet-induced hyperlipidemia resulted in the identification of 19 urine and 16 plasma metabolites as potential biomarkers of its therapeutic effect.
Why the study?
Little was known regarding the in vivo metabolic profiling of Guan-Xin-Shu-Tong capsule, motivating an evaluation of altered metabolic pathways and potential biomarkers in hyperlipidemia.
Does Guan-Xin-Shu-Tong capsule alter metabolic pathways and biomarkers in rats with diet-induced hyperlipidemia?
Does Guan-Xin-Shu-Tong capsule alter metabolic pathways and biomarkers in rats with diet-induced hyperlipidemia?
Guan-Xin-Shu-Tong capsule alters metabolic profiles in rats with diet-induced hyperlipidemia, identifying 35 potential biomarkers in urine and plasma.
May identify biomarkers for Guan-Xin-Shu-Tong capsule; leaves open translation to human hyperlipidemia.
Guan-Xin-Shu-Tong capsule is a widely used traditional Chinese medicine for the treatment of cardiovascular diseases. However, little knowledge about the metabolic profiling in vivo after treating with Guan-Xin-Shu-Tong capsule was reported. To acquire the changed metabolism pathways and search for the potential biomarkers, a metabonomics approach of Guan-Xin-Shu-Tong capsule against hyperlipidemia was developed based on UPLC-MS/MS. A Shimpack XR-ODS C18 column (75 × 3.0 mm, 2.2 μm) was applied for separation at the flow rate of 0.4 mL min−1. Multivariate statistical analysis (OPLS and OPLS-DA) revealed obvious differentiation between the natural group, hyperlipidemia group, positive group and Guan-Xin-Shu-Tong capsule treated group in both positive and negative ion modes. A total of 19 metabolites from urine samples and 16 metabolites from plasma samples were identified as potential biomarkers. Our results showed that such a metabonomics approach could not only provide a systematic view of hyperlipidemia-related metebolism, but also reveal the mechanism of therapeutic effect of Guan-Xin-Shu-Tong capsule in treating hyperlipidemia.
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Gao et al. (2021) studied Diet-induced hyperlipidemia. Guan-Xin-Shu-Tong capsule vs. Natural group, hyperlipidemia group, positive group was evaluated on Metabolic profiling and potential biomarkers. Guan-Xin-Shu-Tong capsule treatment in rats with diet-induced hyperlipidemia resulted in the identification of 19 urine and 16 plasma metabolites as potential biomarkers of its therapeutic effect.
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