Population
Junctional terminal cisternae of fast skeletal muscle sarcoplasmic reticulum (SR)
Comparison
Solubilization with CHAPS and purification by… vs Original SR vesicles
Design
Preclinical
Key result
Purified ryanodine receptor from fast skeletal muscle bound 393 +/- 65 pmol of ryanodine/mg of protein and structurally corresponded to the feet structures of junctional terminal cisternae.
Authors
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Links ryanodine receptor to Ca2+ release in skeletal muscle feet; leaves open human disease relevance and therapeutic targeting.
The study demonstrates that the ryanodine receptor and Ca2+ release channel represent a functional unit, with the structural unit being the foot structure of the junctional terminal cisternae.
Inui et al. (1987) studied this question. Purification of ryanodine receptor was evaluated on Ryanodine binding and structural characteristics. Purified ryanodine receptor from fast skeletal muscle bound 393 +/- 65 pmol of ryanodine/mg of protein and structurally corresponded to the feet structures of junctional terminal cisternae.