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A new method for identification and characterization of an acyl phosphate linkage in phosphorylated proteins is presented. The method involves reductive cleavage of the acyl phosphate bond with sodium 3Hborohydride to form a labeled aminohydroxy acid residue. 3HBorohydride reduction of the phosphorylated (Ca2+, Mg2+)-adenosine triphosphatase of sarcoplasmic reticulum, followed by analysis of the acid hydrolysate of the reduced enzyme, showed the formation of labeled homoserine. The results demonstrate that the phosphoryl group of sarcoplasmic reticulum ATPase is attached to the β-carboxyl group of an aspartyl residue at the active site.
Degani et al. (1973) studied this question.