Comparison of different procedures, transport media, and enrichment media for isolation of Campylobacter species from healthy laying hens and humans with diarrhea

A comparison was made with some of the available transport and enrichment media for the isolation of Campylobacter species. The merits of duplicate versus single sampling were also investigated. Fecal samples from 721 healthy laying hens and 328 patients with diarrhea were included in the study. The media compared were modified Stuart medium, Campythio-glycolate medium, semisolid motility test medium, and Campylobacter enrichment broth. The isolation rate of Campylobacter species was significantly increased by taking duplicate instead of single samples. The highest outcome was found when one of the two samples was an enrichment medium, such as semisolid motility test medium.