Effect of seed inoculation with bacterial inoculum and in-row application of biohumus on germination parameters, physiological and biochemical components of spring barley seedlings under laboratory conditions

A short-term vegetative experiment was conducted at the Kyiv Specialized Branch of the Ukrainian Institute of Plant Variety Examination (Ukraine) to determine the effect of small doses (100–300 kg ha-1) of biohumus applied during sowing in rows with seeds, as well as pre-sowing inoculation of seeds with the BioStim™Seed preparation on germination parameters and physiological indicators of spring barley seedlings. In row application of biohumus, BioStim™Seed, and their combination improved germination response parameters under laboratory conditions. Pre-sowing inoculation of spring barley seeds with preparation BioStim™Seed without adding biohumus increased germination percentage, but at the same time the mean germination time was increased. The combined use of pre-sowing seed inoculation with the BioStim™Seed preparation with row application of biohumus significantly increased morphological parameters of spring barley shoots, chlorophyll a content, the sum and ratio of chlorophylls a and b, carotenoids content, catalase and peroxidase activity, while significantly reducing the MDA content in the leaves of spring barley seedlings.