Immunization of rabbits with lysine vasopressin (LVP) conjugated to bovine serum albumin has resulted in the production of antibodies to LVP which are capable of crossreacting with arginine vasopressin (AVP). Using the antiserum and LVP-126I, a radioimmunoassay for AVP has been developed which is sensitive and specific for vasopressin. The reliability and specificity of the assay are confirmed by performing simultaneous immuno- and bioassays for AVP on the posterior pituitaries of normally hydrated rats and rats subjected to varying periods of dehydration. A highly significant linear correlation is demonstrated when pituitary AVP content by immunoassay (Y) is plotted against pituitary AVP content by bioassay(X) (Y =1.15X +81.05, r =0.941, p<.001). Good agreement occurs for the value of pituitary AVP content as determined by the 2 assay methods, with immunoassay values being somewhat greater than those determined by bioassay. Following prolonged dehydration, there is a progressive parallel decrease in pituitary AVP content as determined by both immuno- and bioassay. It is thus established that the radioimmunoassay for AVP is a reliable, specific and sensitive method, capable of quantitatively measuring biologically active AVP in physiological conditions known to affect pituitary AVP content. (Endocrinology84: 557, 1969)
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Miller et al. (1969) studied this question.