Dietary NiCl2 causes G2/M cell cycle arrest in the broiler's kidney

// Hongrui Guo 1 , Hengmin Cui 1,2 , Xi Peng 1,2 , Jing Fang 1,2 , Zhicai Zuo 1,2 , Junliang Deng 1,2 , Xun Wang 1,2 , Bangyuan Wu 1 , Kejie Chen 1 and Jie Deng 1 1 Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya’an, China 2 College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, China Correspondence to: Hengmin Cui, email: // Keywords : NiCl2, G2/M cell cycle arrest, mRNA expression, protein expression, kidney Received : June 29, 2015 Accepted : September 14, 2015 Published : September 30, 2015 Abstract Here we showed that dietary NiCl 2 in excess of 300 mg/kg caused the G 2 /M cell cycle arrest and the reduction of cell proportion at S phase. The G 2 /M cell cycle arrest was accompanied by up-regulation of phosphorylated ataxia telangiectasia mutated (p-ATM), p53, p-Chk1, p-Chk2, p21 protein expression and ATM, p53, p21, Chk1, Chk2 mRNA expression, and down-regulation of p-cdc25C, cdc2, cyclinB and proliferating cell nuclear antigen (PCNA) protein expression and the cdc25, cdc2, cyclinB, PCNA mRNA expression.