Key result
The Myostation-Intact system successfully detected isolated mouse papillary muscle contractility, which varied with frequency (0.45 to 0.10 mN), voltage, and calcium concentration.
Why the study?
Detecting ex vivo myocardial contractility is necessary to understand the relationship between myocardial contractility and external stimuli.
The Myostation-Intact system provides a viable method for detecting ex vivo myocardial contractility in isolated mouse papillary muscles under varying stimuli.
Facilitates ex vivo mouse contractility assays; leaves open translation to human myocardial assessment.
BACKGROUND: To understand the relationship between myocardial contractility and external stimuli, detecting ex vivo myocardial contractility is necessary. METHODS: We elaborated a method for contractility detection of isolated C57 mouse papillary muscle using Myostation-Intact system under different frequencies, voltages, and calcium concentrations. RESULTS: The results indicated that the basal contractility of the papillary muscle was 0.27 ± 0.03 mN at 10 V, 500-ms pulse duration, and 1 Hz. From 0.1 to 1.0 Hz, contractility decreased with an increase in frequency (0.45 ± 0.11-0.10 ± 0.02 mN). The voltage-initiated muscle contractility varied from 3 to 6 V, and the contractility gradually increased as the voltage increased from 6 to 10 V (0.14 ± 0.02-0.28 ± 0.03 mN). Moreover, the muscle contractility increased when the calcium concentration was increased from 1.5 to 3 mM (0.45 ± 0.17-1.11 ± 0.05 mN); however, the contractility stopped increasing even when the concentration was increased to 7.5 mM (1.02 ± 0.23 mN). CONCLUSIONS: Our method guaranteed the survivability of papillary muscle ex vivo and provided instructions for Myostation-Intact users for isolated muscle contractility investigations.
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Lian et al. (2022) studied Isolated C57 mouse papillary muscle. Myostation-Intact system was evaluated on Myocardial contractility. The Myostation-Intact system successfully detected isolated mouse papillary muscle contractility, which varied with frequency (0.45 to 0.10 mN), voltage, and calcium concentration.
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