What question did this study set out to answer?

To investigate how superoxide and nitric oxide interact to produce peroxynitrite and nitrate tyrosine.

Superoxide Reacts with Nitric Oxide to Nitrate Tyrosine at Physiological pH via Peroxynitrite

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Randomized trial demonstrates the formation of peroxynitrite that nitrates tyrosine, indicating its role at physiological pH.

To investigate how superoxide and nitric oxide interact to produce peroxynitrite and nitrate tyrosine.
Used a stopped-flow spectrophotometer to mix potassium superoxide with nitric oxide at pH 7.4.
Examined the yield of tyrosine nitration in the presence of the reaction intermediates and carbon dioxide effects.
Analyzed the effects of urate from hypoxanthine oxidation on tyrosine nitration when using xanthine oxidase.
Intermediate formed from superoxide and nitric oxide nitrated tyrosine in 100% yield, matching preformed peroxynitrite behavior.
Nitric oxide alone under aerobic conditions did not yield detectable nitrotyrosine.
Urate significantly inhibited tyrosine nitration during reactions with xanthine oxidase.

Reiter et al. (2000) studied this question.

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