August 1, 1974Open Access

Binding of Adenylyl Imidodiphosphate, an Analog of Adenosine Triphosphate, to Myosin and Heavy Meromyosin

Q: What is the clinical evidence from this study?

Study design: Other. Intervention: Adenylyl imidodiphosphate (App(NH)p). Primary outcome: Dissociation constant of App(NH)p with myosin.

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Key Result

The dissociation constant of adenylyl imidodiphosphate with myosin was 0.400 ± 0.040 PM, identical to the previously reported Km value for ATP hydrolysis.

PICO

Intervention / Comparator

Adenylyl imidodiphosphate (App(NH)p)

Primary Outcome

Dissociation constant of App(NH)p with myosin

Abstract

The binding of adenylyl imidodiphosphate (App(NH)p), an analog of ATP with an imido group (-NH-) in place of the terminal oxygen bridge, to myosin and heavy meromyosin was studied.Binding studies were performed at 23-25" in 0.020 M Tris-HCl, 1.00 mM MgS04, and 0.100 M NaCl at pH 7.4.The dissociation constant of App(NH)p with myosin, 0.400 + 0.040 PM, is identical with the previously reported K,,, value for ATP hydrolysis, 0.41 PM (SCHLISELFELD, L., AND

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Authors

Louis H. Schliselfeld

Westchester Medical Center

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Journal of Biological Chemistry

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Westchester Institute for Human Development

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Louis H. Schliselfeld (Thu,) reported a other. Adenylyl imidodiphosphate (App(NH)p) was evaluated on Dissociation constant of App(NH)p with myosin. The dissociation constant of adenylyl imidodiphosphate with myosin was 0.400 ± 0.040 PM, identical to the previously reported Km value for ATP hydrolysis.

synapsesocial.com/papers/6a22fddbc650520b07cb1fd1 — DOI: https://doi.org/10.1016/s0021-9258(19)42417-4

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Binding of Adenylyl Imidodiphosphate, an Analog of Adenosine Triphosphate, to Myosin and Heavy Meromyosin

Key Result

PICO

Abstract

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Discussion

Authors

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References and Citations

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Discussion

Cite this study

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