Key result
Microinjected fluorescent myosin and its light meromyosin subfragment successfully incorporated into the A-bands of myofibrils in living cultured chick myotubes.
Microinjected myosin and its light meromyosin subfragment are capable of associating with physiological myofibrillar structures in living muscle cells.
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Demonstrates myosin incorporation into living myofibrils; leaves open translation to mammalian cardiac muscle in vivo.
Johnson et al. (1988) studied this question. Microinjected fluorescently labeled skeletal muscle myosin and subfragments was evaluated on Incorporation into myofibrillar structures. Microinjected fluorescent myosin and its light meromyosin subfragment successfully incorporated into the A-bands of myofibrils in living cultured chick myotubes.
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