SUMMARY Plaques were produced in mouse-embryo monolayers by infection with polyoma virus DNA in the presence of diethylaminoethyl-dextran. Optimum conditions for plaque assay were established and dose-response relationships for component I and component (II+III) polyoma DNA determined. Efficiencies up to 6 × 105 p.f.u./µg. were obtained for component I DNA.
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Warden et al. (1968) studied this question.
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