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The success of cancer immunotherapy is strongly influenced by the tumor immunophenotype, which is categorized as inflamed, immune-excluded, or immune-desert. However, noninvasively differentiating these key immunophenotypes to predict therapeutic outcomes remains a major challenge. Here, we report a fluorescence-afterglow reporter (FAR) for the real-time, in vivo differentiation of these three immunophenotypes. FAR operates on a dual-signal logic, simultaneously reporting on M1 macrophage polarization via a nitric oxide (NO)-responsive near-infrared fluorescence (NIRF) signal and on tumor cell apoptosis via a Caspase-3-activatable afterglow signal. This logical integration enables the accurate differentiation of inflamed NIRF (ON)-Afterglow (ON), immune-excluded NIRF (ON)-Afterglow (OFF), and immune-desert NIRF (OFF)-Afterglow (OFF) phenotypes in living mice. FAR also sensitively monitored the therapeutic conversion of an immune-excluded tumor to an inflamed state following combination therapy. Notably, FAR's signal patterns, whether from direct tumor imaging or a complementary urinalysis enabled by its renal-clearable design, strongly correlated with therapeutic outcomes, providing early predictive value for immunotherapy efficacy. Thus, this dual-signal logic probe provides functional insights into the tumor immune microenvironment, offering a powerful tool to guide the development and application of personalized cancer immunotherapy.
Li et al. (Tue,) studied this question.