January 1, 1990AJP Heart and Circulatory Physiology
Mechanism of ischemic contracture in ferret hearts: relative roles of Ca2+i elevation and ATP depletion
Key result
ATP depletion, rather than elevation of intracellular free calcium, is the primary mechanism initiating ischemic contracture in ferret hearts.
Population
ferret hearts
Comparison
total global ischemia with or without iodoacetate vs control perfusion conditions
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Does not alter clinical ischemia management; leaves open ATP-targeted strategies pending human validation.
Key Points
- The study aims to differentiate the roles of intracellular calcium elevation and ATP depletion in ischemic contracture in ferret hearts.
- Measured intracellular calcium concentration ([Ca2+]i) and ATP levels using nuclear magnetic resonance (NMR) spectroscopy.
- Observed changes in these concentrations during periods of total global ischemia.
- Examined effects of glycolysis inhibition with iodoacetate on ATP and force dynamics.
- [Ca2+]i increased to between 2-3 microM after 30-35 min of ischemia, while force rose only after 40 min.
- [ATP] dropped to less than 10% of control levels just before the onset of ischemic contracture.
- Inhibition of glycolysis drastically reduced [ATP] and raised force prior to any increase in [Ca2+]i.
Structured PICO
P
Population
Ferret hearts subjected to total global ischemia to study the mechanism of ischemic contracture.
E
Exposure
total global ischemia with or without iodoacetate (an inhibitor of glycolysis)
C
Comparator
control perfusion conditions
O
Outcome
intracellular free calcium concentration ([Ca2+]i), ATP concentration ([ATP]), and diastolic force measured using nuclear magnetic resonance (NMR) spectroscopysurrogate
In ferret hearts, ATP depletion rather than intracellular calcium elevation is the primary mechanism responsible for the initiation of ischemic contracture.
Cite This Study
Koretsune et al. (1990) studied Ischemic contracture. Total global ischemia vs. Control perfusion conditions was evaluated on Intracellular free calcium concentration ([Ca2+]i), ATP concentration, and diastolic force. ATP depletion, rather than elevation of intracellular free calcium, is the primary mechanism initiating ischemic contracture in ferret hearts.