Key result
eNOS gene transfer markedly inhibited AngII-induced Rho/Rho-kinase activation and subsequent vascular smooth muscle cell migration via specific inhibition of G12/13 activation.
eNOS gene transfer prevents Angiotensin II-induced vascular smooth muscle cell migration by inhibiting the G12/13 and Rho/Rho-kinase pathway, revealing a novel mechanism for cardiovascular protection by nitric oxide.
Animal data suggest eNOS may limit AngII vascular effects; leaves open human therapeutic targeting of this pathway.
BACKGROUND: Although, endothelial nitric oxide (NO) synthase (eNOS) is believed to antagonize vascular remodeling induced by the angiotensin II (AngII) type-1 receptor, the exact signaling mechanism remains unclear. METHODS AND RESULTS: By expressing eNOS to vascular smooth muscle cells (VSMCs) via adenovirus, we investigated a signal transduction mechanism of the eNOS gene transfer in preventing vascular remodeling induced by AngII. We found marked inhibition of AngII-induced Rho/Rho-kinase activation and subsequent VSMC migration by eNOS gene transfer whereas G(q)-dependent transactivation of the epidermal growth factor receptor by AngII remains intact. This could be explained by the specific inhibition of G(12/13) activation by eNOS-mediated G(12/13) phosphorylation. CONCLUSIONS: The eNOS/NO cascade specifically targets the Rho/Rho-kinase system via inhibition of G(12/13) to prevent vascular migration induced by AngII, representing a novel signal cross-talk in cardiovascular protection by NO.
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Suzuki et al. (2008) studied Vascular remodeling. eNOS gene transfer via adenovirus was evaluated on AngII-induced Rho/Rho-kinase activation and subsequent VSMC migration. eNOS gene transfer markedly inhibited AngII-induced Rho/Rho-kinase activation and subsequent vascular smooth muscle cell migration via specific inhibition of G12/13 activation.
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