A two-phase system in vitro has been developed for studying the bone-mobilizing effects of parathyroid hormone. Phase I consists of the incubation of hormone with ascites cells, and Phase II is the incubation of Phase I supernatant with dead bone powder. The mobilization of labeled calcium from this powder is the index of hormonal activity. The response to hormone is apparent at 10-8 m hormone, appears specific for parathyroid hormone, and has striking analogies to hormone action upon living bone in vivo and in vitro.
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Tenenhouse et al. (1966) studied this question.
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