Key result
Takotsubo syndrome activates myocardial necroptosis and pyroptosis pathways in preclinical models.
Why the study?
Takotsubo syndrome is associated with an inflammatory response, prompting evaluation of the presence and dynamics of necroptosis and pyroptosis in a female rat model.
Does isoprenaline induce inflammatory-associated programmed cell death in a female rat model of Takotsubo syndrome?
Does isoprenaline induce inflammatory-associated programmed cell death in a female rat model of Takotsubo syndrome?
This experimental study suggests that inflammation in Takotsubo syndrome may be primarily driven by programmed cell death pathways (necroptosis and pyroptosis).
Answers are grounded in this paper and cite their sources.
Should not alter Takotsubo management; hypothesis-generating for necroptosis and pyroptosis as inflammation drivers.
Takotsubo syndrome (TTS) is associated with inflammatory response, therefore the aim of the study was to evaluate the presence and dynamics of inflammatory-associated forms of cell death, necroptosis, and pyroptosis in the female rat model of isoprenaline (ISO)-induced TTS. TTS was induced in female Sprague Dawley rats (n = 36) by ISO 150 mg/kg intraperitoneally. Animals were divided into four groups: TTSO (TTS+ovariectomy; n = 10), TTSP (TTS+sham operation; n = 10), CO (0.9% NaCl+ovariectomy; n = 8), CP (0.9% NaCl+sham operation; n = 8). Histopathological analysis, evaluation of plasma concentration, and myocardial expression of pyroptosis- and necroptosis-associated proteins were performed. TTSO and TTSP groups had higher plasma concentrations of interleukin-1β in comparison with the controls. Low myocardial protein expression of mixed lineage kinase domain-like pseudokinase (MLKL), caspase-1 (Casp-1), and calcium/calmodulin-dependent kinase type II isoform delta (CAMKIIδ) was visible 6 and/or 12 h post-ISO. Twenty-four hours post-ISO, high myocardial and vascular protein expression of CAMKIIδ was visible in TTSO but not TTSP rats, while high myocardial expression of MLKL and Casp-1 was visible both in TTSO and TTSP rats. The course of TTS is associated with activation of inflammatory-associated programmed cell death, necroptosis, and pyroptosis, therefore inflammation may be a primary response occurring simultaneously with cardiomyocyte death in TTS.
No takes yet. Share an insight, caveat, or question.
Borodzicz-Jażdżyk et al. (2023) studied Takotsubo syndrome model (n=36). Isoprenaline vs. 0.9% NaCl was evaluated on Myocardial expression of pyroptosis- and necroptosis-associated proteins and histopathological changes. Isoprenaline-induced Takotsubo syndrome in female rats was associated with activation of necroptosis and pyroptosis pathways, evidenced by elevated plasma interleukin-1β and high myocardial expression of MLKL and Caspase-1.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: