Key result
Endothelin-1 (100 nM) increased twitch cell shortening in 44% of rat ventricular myocytes, an effect driven by increased Ca2+ transient amplitude rather than myofilament responsiveness.
Population
Rat isolated ventricular myocytes loaded with a fluorescent Ca2+ indicator indo-1 (n=14 cells)
Design
Preclinical
Follow-up
30 min
Authors
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Heterogeneous ET-1 inotropy in rat myocytes warrants no clinical extrapolation; leaves open Ca2+-mediated mechanisms for human validation.
The positive inotropic effect of endothelin-1 in rat ventricular myocytes is heterogeneous and mediated primarily by increased Ca2+ transient amplitude rather than altered myofilament responsiveness.
Katoh et al. (1998) studied this question. Endothelin-1 (ET-1) was evaluated on Twitch cell shortening and Ca2+ transient amplitude. Endothelin-1 (100 nM) increased twitch cell shortening in 44% of rat ventricular myocytes, an effect driven by increased Ca2+ transient amplitude rather than myofilament responsiveness.
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