Protocol demonstrates enhanced viability and structural integrity in cortical organoids, indicating improved neurodevelopmental modeling.
Cortical organoids (COs) derived from human induced pluripotent stem cells (hiPSCs) provide a unique opportunity to model human neurodevelopment in vitro. Here, we present a protocol to prevent necrotic core formation without requiring organoid slicing. We describe steps for hiPSC culturing, preparation of single-cell suspension, seeding, and embryoid body (EB) and neuroectoderm formation. We then detail procedures for CO formation and maintenance without necrotic core formation. This protocol sustains long-term viability and preserves structural integrity by enhancing oxygen and nutrient diffusion.
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Mamun et al. (2026) studied this question.
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