1009-OR: Dual Antibacterial and Regenerative Effects of Autologous Nanofat-PRF Membranes: A Translational Strategy for Diabetic Foot Ulcer Management

Introduction and Objective: Diabetic foot ulcers (DFUs) are a major cause of morbidity and lower-limb amputation in people with diabetes, driven by impaired tissue regeneration and high susceptibility to bacterial infection. Current management relies heavily on systemic antibiotics, with limited impact on healing and rising antimicrobial resistance. Autologous regenerative therapies combining platelet-rich fibrin (PRF) and adipose-derived nanofat may offer a tissue-based strategy addressing both regeneration and infection control. This study evaluated the antibacterial activity of autologous nanofat-PRF membranes against clinically relevant pathogens and their translational relevance for DFU care. Methods: An in vitro experimental study was conducted using autologous nanofat and injectable PRF obtained from healthy donors. Composite nanofat-PRF membranes with different ratios were tested against DFU-associated pathogens (Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Enterococcus faecalis) using the Kirby-Bauer disc diffusion method. Gentamicin and ampicillin served as positive controls, while injectable PRF alone and blank discs were negative controls. Antibacterial activity was quantified by inhibition zone diameter and analyzed using ANOVA (p < 0.05). Results: Nanofat-PRF membranes demonstrated significant antibacterial activity against all tested pathogens. P. aeruginosa showed the highest susceptibility, whereas S. aureus exhibited the lowest inhibition response. No significant differences were observed among membrane compositions. Injectable PRF alone showed no antibacterial effect. Conclusion: Autologous nanofat-PRF membranes display a dual antibacterial and regenerative profile targeting key drivers of diabetic foot ulcers. These findings support their potential role as an adjunctive, antibiotic-sparing strategy in complex diabetic wounds and provide a strong translational rationale for future clinical study. Disclosure P. Martin-Marfil: None. N. Fakih-Gomez: None.