A New Sulfated Cholestane From the Sea Urchin Mesocentrotus nudus With Cytotoxic Activity

A new sulfated cholestane derivative, 5 α ,8 α ‐epidioxy‐cholesta‐6‐en‐3 β ‐ O ‐sulfate ( 1 ), together with three known sterols, cholest‐7‐ene‐3,5,6‐triol ( 2 ) and a mixture of 24( S )‐hydroxy‐24‐vinylcholesterol ( 3 ) and 24( R )‐hydroxy‐24‐vinylcholesterol ( 4 ), was isolated from the viscera of the sea urchin Mesocentrotus nudus . The structures were elucidated by comprehensive spectroscopic analyses (1D and 2D NMR and HRESIMS) and by comparison with reported data. Compound 1 exhibited moderate cytotoxicity against K562 human leukemia and SW480 human colon cancer cells, with IC 50 values of 16.72 ± 0.28 µM and 17.79 ± 0.32 µM, respectively. This work represents the first report of a 5 α ,8 α ‐epidioxy sterol from the genus Mesocentrotus and adds to the chemical diversity of sea urchin sulfated sterols, providing a basis for further biological evaluation.